Deazaflavin Powder

Healthkintai^® is a manufacturer and supplier of deazaflavin. Deazaflavin (CAS No. 26908-38-3) is a structural analogue of flavin cofactors, characterized by the replacement of one or more nitrogen atoms in the natural flavin molecule with carbon atoms. The most common type, 5-deazaflavin, is a derivative that replaces the N5 position in the flavin ring with the C5 position. Deazaflavin Powder has applications spanning multiple fields, including biomedicine, cosmetics, and dietary supplements. With its unique chemical structure and multifunctional biological activities, deazaflavin is becoming a promising raw material ingredient in the pharmaceutical, health product, and functional cosmetic fields. Contact us at info@kintaibio.com.

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Condensation Reaction

Charge barbituric acid and 2-nitrobenzaldehyde into the reactor at a 1:1.2 molar ratio, add water and isopropanol as mixed solvent, heat to 82°C, and reflux for 2-6 hours to generate the yellow benzylidene barbituric acid intermediate. After the reaction is complete, cool down, filter under suction, and dry to obtain a yellow solid powder with a yield of approximately 86%.

Reduction and Cyclization

Add the intermediate from Step 1 to a mixed solvent of water and tetrahydrofuran, heat to 70°C, and add a sulfide reducing agent in batches to reduce the nitro group to an amino group, which immediately undergoes nucleophilic attack to complete cyclization and form the core deazaflavin skeleton. After approximately 5 hours of reaction, concentrate, filter under suction, and wash with water to obtain the crude product.

Purification

Add the crude product to isopropanol as solvent, heat to 88°C, and stir for 3 hours to slurry. Filter hot to remove insoluble impurities, then cool to crystallize. Separate the crystals by suction filtration and dry to obtain a white pure powder with purity exceeding 99% .

Testing and Packaging

Sample the dried, purified deazaflavin powder and perform quality testing, including HPLC for purity (≥99% required), LC-MS for molecular weight confirmation, Karl Fischer titration for moisture content (≤0.5% required), along with heavy metals residue and microbial limits testing.

Desoxyflavin exhibits some solubility in water and various organic solvents. In water, its solubility is approximately 1683 mg/L (25°C), but this is significantly affected by pH and temperature, falling within the range of soluble to partially soluble. In practical applications, for high-concentration aqueous solutions, appropriate heating or pH adjustment can be performed. In organic solvents, desoxyflavin is soluble in dimethyl sulfoxide, methanol, ethanol, and pyridine. For formulation development or experimental applications, it is recommended to first conduct experimental testing with a small sample, or prepare a high-concentration stock solution using DMSO followed by dilution with buffer solution to obtain ideal solubility results.

Deazaflavin for Alzheimer's disease

This is currently the most promising area of ​​efficacy for desoxyflavin. Studies have shown that the it derivative TND1128 is a highly effective mitochondrial activator that can significantly improve neuronal energy metabolism. Hydrogen peroxide (H₂O₂)-induced oxidative stress is a significant factor leading to mitochondrial dysfunction and neurodegenerative diseases. Research has confirmed that TND1128 and its analogues can significantly improve cell survival under oxidative stress damage, exerting cytoprotective effects by activating mitochondria, demonstrating potential as a treatment for neurodegenerative diseases such as Alzheimer's disease.

Deazaflavin for Skin

The skin benefits of desoxyflavin primarily focus on its anti-aging and cell repair properties. As a novel vitamin-like compound, desoxyflavin can effectively activate mitochondrial function, promote intracellular DNA repair, and possess significant antioxidant effects. By activating the NAD+ pathway, which is closely related to energy metabolism, desoxyflavin can help maintain the health of skin cells, improve skin texture, and alleviate age-related skin problems such as sagging and dullness.

Deazaflavin for anti-aging

The anti-aging effects of 5-Deazaflavin are mainly concentrated in three aspects: mitochondrial repair, activation of longevity proteins, and antioxidation. 5-desaturated flavin derivatives, represented by TND1128, can efficiently activate mitochondrial energy synthesis, significantly improve cell damage caused by oxidative stress, and have the potential to repair neurons that are functionally degenerating in aging or disease. Regarding the activation of deacetylases (Sirtuins), 3-methyl-10-ethyl-desaturated flavin (M10EDF) can directly enhance the activity of SIRT1/3/6 through allosteric effects, with an efficiency far exceeding that of the traditional NMN and NAD+ pathways.

Deazaflavin pharmaceuticals

In the pharmaceutical field, the dosage form design of 5-Deazaflavin mainly revolves around two main directions: oral solid dosage forms and injections. Oral solid dosage forms are usually the most widely used, with capsules being the most common. Regarding injections, due to the solubility of desoxyflavin in both water and organic solvents, lyophilized powder injections are relatively common. This dosage form facilitates maintaining the drug's stability during storage, and it is reconstituted into an injection solution for clinical use, often used in adjuvant cancer therapy or neuroprotective applications. In the field of topical drug delivery, transdermal absorption formulations (such as liposome gels or patches) are also a dosage form worth noting for skin repair or treatment of localized neuropathy.

Deazaflavin Supplements

In the health supplement industry,Deazaflavin Powder products are already quite diverse, covering mainstream dosage forms such as capsules, tablets, powders, and functional food beverages. Among these, plant-based capsules are the most common form; for example, there are 150-capsule vegetarian capsules on the market, each containing 60mg of pure desoxyflavin, emphasizing a research-grade, filler-free, pure formula. In addition, functional beverages are also an important application area, such as dietary drinks fortified with desoxyflavin or ready-to-drink liquid supplements. It is often used in combination with other anti-aging ingredients such as NMN to create compound tablets.

Deazaflavin in skincare

In the cosmetics industry, the formulation design of desaturated flavonoids focuses on improving the stability of active ingredients and transdermal absorption. Serums and freeze-dried powders are currently the most mainstream application forms. Desaturated flavonoids are also beginning to appear in anti-aging creams and eye repair serums. These products typically combine them with moisturizing ingredients such as hyaluronic acid to create a comprehensive repair system. Freeze-dried masks or powdered beauty serums are also promising formulations, as the on-demand preparation method minimizes the degradation of active ingredients in aqueous solutions.

Regarding the safety of Deazaflavin, current data primarily come from in vitro experiments and evaluations as a research product. Existing studies indicate that this compound itself exhibits low inherent cytotoxicity. In studies investigating its use as an adjuvant anticancer drug, some desoxyflavin derivatives (such as ZW-1288) showed only weak cytotoxicity when used alone, indicating relatively good basic safety. However, it is particularly noteworthy that these compounds can significantly enhance the cytotoxic effects of other drugs (such as etoposide), suggesting a possible synergistic effect through influencing DNA repair pathways.

Choosing our Deazaflavin Powder guarantees you triple assurance in quality, technology, and service. First, regarding quality control, we implement rigorous testing at every step, from raw material warehousing to finished product delivery, ensuring product purity of over 99% and excellent batch-to-batch stability. Second, in terms of technical support, our experienced R&D team provides comprehensive assistance from solubility testing to formulation development, helping you solve practical problems such as dosage form design and stability optimization. Finally, regarding supply chain security, we possess stable production capacity and flexible supply solutions, enabling rapid response to everything from sample trials and small-order pilot production to large-scale mass production. Contact us at info@kintaibio.com.

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